![]() Then, while you're signed in at, the "Your Digital Items" menu in the upper right of the Amazon page structure gives you access the Cloud Drive and related services. The only software you'll need to use your Cloud Drive is a Web browser running the popular, free Adobe Flash software. It's like having a USB flash drive that's accessible from anywhere on the Internet.Īmazon Cloud Drive is available to any user with a valid billing address. In addition, you can download those files again anywhere you're able to log in to. ![]() With the Cloud Drive, you can upload files to the cloud and organize them via a user-friendly interface. The Amazon Cloud Drive is the storage service behind those products. Using the power of those same cloud resources, though, Amazon has developed new products you can use for both play and work. While AWS has many services that are useful to the business world, those services may not be very useful to the average Amazon user. This article describes how Amazon Cloud Player works, including the Amazon Cloud Drive powering it, and some of the challenges Amazon is facing in offering its downloadable MP3s and streaming services from cloud storage. The first 5 GB of storage is free, and mobile devices with the Amazon MP3 Player can stream music online so you can listen to it straight from the Internet instead of downloading it to your device. The Amazon Cloud Player lets you lease disk space from Amazon's cloud to store your music and other files and to access them from anywhere. ![]() The Amazon Cloud Player is focusing that cloud power for Internet-connected personal stereos. You'd never had to remove old files just so you can add new ones.Ĭloud computing is making such seemingly infinite storage a reality for all kinds of computing uses. It's like having an iPod with an infinite amount of storage space. Now imagine that you could store all the music you have now, plus all the music you'll ever buy, as MP3 files, and that you'd never have to worry about whether you have enough space to store your whole collection. Unfortunately, the cards, like iPods, can still only store so much at one time. This eliminates the need to repeatedly upload and erase files you just switch out the card when you want different music. ![]() Some manufacturers have produced players that use removable media such as SD cards. While personal stereo capacities have increased, they still have finite storage space. In its first ten years, iPod models went from 5 GB to 160 GB in capacity, letting you store days worth of music on a single, small device. In 2001, Apple released just such a device: the iPod. With the invention of new digital file formats like MP3, the next logical step for personal stereos was storing and playing music as digital files the same way you would on a computer hard drive. Audio cassettes only held up to two hours of music, and CDs about an hour and a half. For several years, the brand name Walkman was as popular as the name iPod today, being as synonymous with personal stereos as Kleenex is to facial tissue.Īs technology progressed, so did the storage capacity of the media used in personal stereos. If you had a Walkman, you could take your favorite tunes with you anywhere in a small, lightweight package. As simple as it was, the device revolutionized the way people listened to music. The original Walkman was a handheld, battery-powered cassette player with stereo headphones. On July 1, 1979, Sony Corporation began selling the world's first personal stereo device, the Walkman.
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![]() There are a lot of file sync softwares that can help you make the robust performance of your data while working anywhere. File sync software works by seamlessly backing up and syncing your data, including your documents, contacts, and calendars, across multiple locations and devices. This is where file sync software can simplify that process. But dealing with data can be a time-consuming and challenging task. Today's businesses are busy, and data is valuable. Companies today are using this cloud computing technology to enable collaboration among teams and have unleashed the power of collaboration around the globe. File sync software is rapidly gaining traction among the masses. With a variety of file sync software available, there is a chance you are making the wrong decision. If you are in the market for file sync software and don't know what to choose, you can be overwhelmed with options. What are the current trends in file sync software? Besides the benefit of the file-sharing feature, they offer many other great features and can be highly beneficial to those who spend a lot of time and effort managing files.Ħ. Now, when you set up File Sync on your iPhone or iPad, which syncs all the files you have saved to the cloud, it can turn on all of your previous backup services.Ī file sync can significantly relieve your day-to-day computing issues. You need to install and sync services on outside machines like your phone and computer. ![]() Besides the benefit of the file-sharing feature, they offer many other great features and can be highly beneficial to those who spend a lot of time and effort managing files. Just imagine if you could save the professional hours of your hardworking employees every day.Ī file sync can significantly relieve your day-to-day computing issues. ![]() But it's an even better idea when it comes to innovative business practices. Saving time has always been great for any business. Slowly technologies are changing our lives, driving down the time we spend with so many tedious tasks, and enabling us to focus on what we like doing - what drives us. How can file sync help in saving your time? You'll need to locate a data storage partner with an open API and a simple transition system for this.ģ.
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![]() Ravengate Partners is a consulting firm dedicated to providing businesses and not-for-profit institutions with education and advice about the financial markets and the global economy. After football Johnny had a newsagent’s business in Bradford before retiring in the 1980s to Tynemouth near Newcastle, where he died in February 2013.Patricia Chadwick is founder and president of Ravengate Partners LLC. Downie then saw out his playing days on the Manchester non-league scene, hanging up his boots in 1962. After switched to Wisbech Town in september 1957, Downie eventually returned to the Football League in October 1958 and played a season at Mansfield Town then spent 1959/60 season at Darlington. Johnny joined Luton Town for a £10,000 fee and scored 12 times in 26 league appearances during the 1953/54 season, including a hattrick on his Hatters’ debut.ĭownie dropped into the non-league scene in the 1955 season, spending two seasons at King’s Lynn although Hull City retained his Football league registration and couldn’t agree a deal for Downie to join Rotherham United in July 1956. When Downie left Old Trafford in July 1953 he had scored 37 times in 116 appearances. In 1952/53 Downie played only 23 times for Manchester United as a number of young and talented players – the Busby Babes – came to prominence. He was the Red Devils’ first choice inside forward for much of the next three seasons, scoring 28 goals including 11 in 1951/52 season when United lifted the First Division title and the Charity Shield – in the latter, Downie scored in a 4-2 win over Newcastle United. In 1944 Downie returned to Bradford Park Avenue and turned professional, between 19 Johnny scored 33 times in 86 starts and his form in 1948/49 season encouraged First Division Manchester United to pay £18,000 for his services in March 1949.ĭownie scored on his Manchester United debut, then netted five goals in 12 starts during the remainder of the 1948/49 season. He was quickly conscripted to become a Bevin Boy, working a miner at Fryston Colliery near Castleford and playing football for the colliery team alongside post-war England international forward Len Shackleton. He came to prominence at 17 playing for the Lanark Air Training Corps XI and signed amateur terms with Bradford Park Avenue in 1942, moving to Yorkshire. John Dennis Downie was born in Lanark in central Scotland, his teenage years were dominated by World War Two. Two wins in April 1955 were enough to save City from relegation, but by that time Brocklebank had dropped Downie, and Johnny left the Tigers in the 1955 close season. In February Downie was withdrawn to a wing half role but that did not halt the decline in City’s form and Jackson was given leave of absence in mid-March to be replaced by Bob Brocklebank. However that Liverpool game was the first of 10 winless League fixtures that saw the Tigers hurtle down the table. ![]() ![]() Downie scored his third City goal in a late October 5-2 win against Notts County, and followed up with another goal a week later at Liverpool. Downie slotted into the inside right position vacated by the injured Syd Gerrie and in late August he opened his City goal account with a brace at home to Lincoln City, and 4-0 win that left City in the top six. Scottish inside forward Johnny Downie joined Hull City in July 1954 as manager Bob Jackson assembled a squad to climb out of the Second Division and complete the job started by his predecessor Raich Carter five years earlier. ![]() Take a break for a few days every two weeks. You shouldn’t take magnesium supplements constantly. Check with your doctor if you take any medications to determine potential interactions. Taking it with food may reduce any abdominal discomfort. You may wish to start with a lower dose and gradually increase to see how your body reacts. Side effects include stomach and intestinal issues. You may also add 1 cup of magnesium flakes to your evening bath, allowing the magnesium to be absorbed through your skin. You may choose to divide your doses between the morning and evening or take your dose before bed. Men may take up to 400 mg daily, and women can take up to 300 mg daily. During this time, researchers found that participants experienced fewer symptoms of insomnia and improved sleep patterns. Participants in a 2012 study took 500 milligrams (mg) of magnesium daily for 2 months. This is thought to encourage healthy sleep patterns. It can help muscles relax and relieve stress. Magnesium is a naturally occurring mineral. If you feel the chanting is causing any ill effects or agitation, stop the practice. You might choose another mantra to use in the daytime. Feel free to recite your mantra as often as you like. Gently bring your mind back to the mantra each time it wanders. A good mantra will allow you to continually focus on the repetition of sound, which will enable you to relax and go to sleep.Ĭhant the mantra mentally or aloud, keeping your focus on the words. It should be a simple, positive statement in the present tense. Choose a mantra that you find pleasant and calming. Search online for ideas or create one that feels right for you. You may choose a mantra in Sanskrit, English, or another language. Participants who continued to use the mantra over the course of a week experienced reduced levels of insomnia. Researchers in a 2015 study taught women who are homeless to repeat a mantra silently throughout the day and before sleeping. Mantras are said to produce feelings of relaxation by quieting the mind. Repeating a mantra or positive affirmation repeatedly can help focus and calm your mind. If you feel it is causing you further angst or turmoil, discontinue the practice.Ĭheck out: The best mediation apps of the year » Meditation is safe to practice, but it has the potential to bring up strong emotions. You may also choose to do an online guided meditation. Consider joining a meditation group once a week to stay motivated. If you don’t have time for a longer session, aim to do 15 minutes in the morning or evening. Participants attended a weekly meditation class, a daylong retreat, and practiced at home over the course of a few months. Researchers in a 2011 study found that meditation significantly improved insomnia and overall sleep patterns. It’s said to reduce stress, improve concentration, and boost immunity. Mindfulness meditation has numerous health benefits that go hand-in-hand with a healthy lifestyle promoting good sleep. You observe your breath, body, thoughts, feelings, and sensations as they rise and pass. Mindfulness meditation consists of slow, steady breathing while sitting quietly. ![]() And to take these great people we have and to push them and make them even better, coming up with more aggressive visions of how it could be. My job is to pull things together from different parts of the company and clear the ways and get the resources for the key projects. Here's how he described his management approach. One of the reasons why Steve Jobs was able to grow Apple into one of the world's most innovative and valuable brands was because he pushed people. Work with someone who pushes you to the extreme. Even having three cofounders isn't too many, assuming you have a clear decision-making hierarchy.Ĭofounders can provide the skills you lack, and take you further than you ever expected you'd go. VCs are more likely to invest in a startup that has a founding team, not a founding individual. It took the inspiration and skills of a cofounder. For me, starting a business took more than just hard work and passion. I couldn't have founded my businesses without my cofounder Hiten Shah. Don't let the specific arrangement get in the way of getting stuff done. The point is, you need to find a way to get the talent to provide their services. Instead of hiring employees, hire on a contract basis. Besides, few people will be willing to take the plunge to become the employee of a tenuous startup. ![]() It may, in fact, form such a barrier that it slows down the process of your startup. Hire contract workers.īecoming an employer carries with it a lot of baggage. Be willing to hire remote workers to get great work done. If you want to find the best and most affordable talent, you may not find it next door. Your business needs more help, knowledge and professional skills than you have time for. Then, when this expert gives you advice - whether business best practice, manufacturing locations, logo design, accounting, whatever - ask that person to do it. When an issue arises, and you don't have the answer, find someone who does. Payment? You can reward the attorney with stocks or deferred payment. Instantly, you will have gained an expert who is implementing his/her own advice for your money. But, then what? Ask the attorney to do it for you. The attorney will provide advice - say it's to start an S-Corp. To get these answers, ask a competent attorney. Here's how he described what happened next: So, he chartered a private flight, despite his lack of money to pay for it. ![]() He was headed to the Virgin Islands for an, um, romantic interlude. ![]() Richard Branson, after all, launched Virgin Airlines in desperation. You can take someone else's product and sell it.
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![]() We tested the efficiency of the standard protocol with several chloroform-to-oil ratios (Chl:O), which were achieved by increasing or decreasing the chloroform volume and keeping other conditions identical. These steps were followed by a high-speed centrifugation to separate the phases into a lower oil phase and an upper aqueous phase, with the latter containing the DNA. Briefly, this protocol consists of combining the emulsion replicates, removing excess oil and homogenizing the sample with Tris-EDTA buffer (10 mM Tris, 1 mM EDTA, pH 8.0) and chloroform (Sigma-Aldrich, MO, USA). Our reference method for DNA retrieval was the chloroform protocol described in the Bio-Rad ddPCR application guide. The method efficiency was calculated as the percentage of DNA recovered against the initial DNA input for each sample ( Supplementary Table 1). The initial and recovered DNA was quantified using the Qubit dsDNA high-sensitivity assay (Thermo Fisher Scientific, MA, USA). Up to three independent emulsion samples were combined into one tube to form a single replicate the number of replicates per protocol in each experiment varied from one to seven. Later, purified PCR amplicons or Qubit ® dsDNA HS Standard #2, of sizes 263 bp and 1 kb, respectively, were chosen. Initially, GeneRuler 100-bp DNA ladder (Thermo Fisher Scientific, MA, USA) was used as the DNA control, containing fragment sizes ranging from 100 to 1000 bp. To assess the efficiency of DNA recovery, DNA fragments up to 1 kb were added to the ddPCR master mix as input. The ddPCR emulsion samples were prepared with QX200 ddPCR EvaGreen Supermix and QX200 Droplet Generation Oil for EvaGreen according to the manufacturer's instructions. Iv) Transfer to silica column and proceed according to manual Iii) Homogenize by vortexing at top speed for 1 min Silica column (GeneJET PCR purification kit, Thermo Scientific) Iv) Homogenize by vortexing at top speed for 1 min Summary of methods used for breaking the ddPCR emulsion. Different chemical and physical methods were compared: chloroform, n-Octane and 1H,1H,2H,2H-perfluoro-1-octanol (PFO) as chemical treatments, and freeze-thawing and silica columns as physical methods ( Table 1). In this study we addressed the challenge of recovering DNA from droplets generated by the QX200™ ddPCR System (Bio-Rad, CA, USA). The lack of efficient methods for breaking the ddPCR emulsion forms a major challenge to using the PCR products in downstream analysis. For instance, DNA recovered from droplets has been used for production of next-generation sequencing libraries for single-cell genetics and transcriptomics studies. ![]() ![]() After the ddPCR process, the droplets are usually discarded, despite their potential as material for downstream genetic studies. However, ddPCR analysis is based on the end-point fluorescence intensity measured for each separate droplet, thereby increasing method sensitivity. As with quantitative PCR, the increase in DNA copy number due to specific amplification is revealed via fluorescent dye. ddPCR relies on DM's generation of numerous nanoliter-sized droplets, inside which independent DNA amplification reactions are carried out. The Droplet Digital™ PCR (ddPCR™) system is a droplet-based PCR platform commercialized by Bio-Rad (CA, USA) and has been widely used for pathogen detection, food quality analysis, environmental studies and medical research. Nonetheless, the most popular and well-established use of DM has been for nucleic acid detection and quantitation. Lately, DM has even been explored for studying single cells. Methods have also been developed to select and cultivate combinations of organisms for studying cell–cell interactions. The replication of microbes inside droplets has revolutionized the study of metabolism, antibiotic resistance and enzyme activity. As a result, droplets can be manipulated in a controlled manner for different ends.ĭM has been used for various purposes ranging from molecular analyses to the cultivation of individual microbes. The properties of the droplets, such as size and stability, can be adjusted by altering the microfluidic channel geometry, flow rate and reagent composition. It relies on the physicochemical properties of two immiscible liquids and their manipulation through interconnected microfluidic channels, enabling the generation and manipulation of small and separated volumes. Droplet microfluidics (DM) technology has proved to be a unique and versatile tool for a broad range of biological assays. ![]() There's been a lot of discussion over the past year, mostly thanks to Epic, about the minimum feature set a digital storefront should provide. Obviously, a slower drive will take much longer to complete the installation. Fun fact: on a new X570 motherboard with PCIe Gen4 SSD, I saw sustained read/write speeds of 1.5GB/s for about a minute while Gears 5 installed. You should see a progress bar across the top of the PowerShell screen indicating installation progress. ![]() Open a PowerShell prompt (Win X and then "I" should do it), navigate to the correct folder ("cd $env:USERPROFILE\Downloads"), and then run the PowerShell cmdlet add-appxpackage with the file (so "add-appxpackage Microsoft.HalifaxBaseGame_1.1.56.0_圆4_8wekyb3d8bbwe.msixvc" for Gears 5). ![]() ![]() Once the download was finished, I ended up with a single massive file, named "Microsoft.HalifaxBaseGame_1.1.56.0_圆4_8wekyb3d8bbwe.msixvc", but what do you do with this? The final step is to install the application via PowerShell. Many games are quite large-Gears of War 5 checks in at 66GB-so this can take a while. Again, from the Reddit thread, I used Firefox with the DownThemAll extension. Anyway, grab that URL and then you'll probably want to paste it into a file downloading utility. I did this for Gears of War 5 and got a link that was at, but it will vary by region and game. In Fiddler, you should see a bunch of requests show up for a Microsoft URL of some form. Install Fiddler and get it running, and then start the download of whatever game/app you want on the Windows Store. First, you need an application to monitor network traffic, and the free utility Fiddler is recommended. This old Forza Horizon 3 Reddit thread has the details, which I'll summarize here. That might not be such a horrible thing if you're dealing with Candy Crush Saga, but the bigger games like Forza, Sea of Thieves, and Gears can easily register at more than 50GB.īut! There is a sort of workaround if you haven't yet downloaded the game and you know you want to have it on multiple PCs. Your only option is to download the entire game, again, on another PC. If you've already downloaded a game in the Windows Store, you're pretty much out of luck. The Microsoft Store won't recognize the files and it won't have the appropriate license. But even if you do take ownership and give your account full access rights-on both the source and destination PCs-and then copy the files over, there's a problem. The files themselves are initially owned by the TrustedInstaller user, and you probably don't want to take ownership as it can screw things up. Or at least, I couldn't get it to work after much effort. Do you want to transfer an existing game download from one PC to another, without downloading the game again? Too bad! You can't do it. Microsoft Store: And finally, the black sheep of digital software platforms known as the Microsoft Store (or formerly, Windows Store). Yes, this is a pain in the butt compared to Uplay or GOG, but at least it's possible. Whether your cloud saves will come along for the ride is another story. It can take several minutes on a slower drive to validate the files, but eventually the game should be available to play. You should see the game begin validation of files-open Task Manager (Ctrl Alt Esc) and look at the Performance tab, and your network connection should show very little activity while your storage drive (C:, D:, or whatever) should show a heavy workload. You want the files and folders from within the game folder, and not the root game folder-so as an example for me, that's the files in "C:\Games\Epic Games\Control" that has 69 items in the root folder, including four folders.įinally, once the file copy is finished, restart the Epic Games Launcher, go to the Library, and click resume on the game. Basically, find the game folder on the new PC where EGS started to download the files, and look for an ".egstore" folder, and then within that should be an "Installation" folder. Now, copy the files from the original PC location (mine are in D:\Games\Epic Games, but the default is in "C:\Program Files"), but the destination folder may vary a bit. Once the install/download process starts, let it grab the first 20MB or so and then pause it, and then exit the Epic Games Launcher. Go to your library, find the game you want to transfer from the other PC, and click the Install button. First, get the Epic Games Launcher installed on the new PC, log in, and get the confirmation email code for your account (which can sometimes take several minutes to arrive). Epic Games Launcher: This one will involve more work, but if you're familiar enough with PCs and Windows Explorer, it's possible to skip a full second (or third…) download of any game on EGS. ![]() Introducing DPIs 4th Generation of their light-weight and compact (LWC) 620W Series battery. Available in 24 volt input to 24 volt output: For 24 volt engines to charge a 24 volt inverter or bow thruster bank. Please read the description below to order a battery charger.Available in 12 volt input to 12 volt output: For 12 volt engines to charge a 12 volt house bank.Available in 24volt input to 12 volt output: For 24 volt engines to charge a 12 volt electronics or house bank.Available in 12 volt input to 24 volt output: For 12 volt engines to charge a 24 volt bow thruster bank.Can charge GEL, AGM or Flooded Lead Acid batteries.Four Step Battery Charging Profile extends the life of your batteries by ensuring a full charge & conditioning, as well as entering a float mode when charging is complete to protect against overcharging your batteries.Recover your batteries up to five times faster than a standard alternator.This allows the flexibility of charging of your auxiliary batteries while underway or while your engine battery charger is plugged in at the dock.Can anyone give me a more reliable firm number Tanks Guys. ![]() By monitoring and then controlling the charge level of the engine battery connected to its input side, the Battery-to-Battery charger is able to provide the output batteries a four-step charging algorithm at amperage levels near full alternator output capacity. I went to a major charger manufacturers web site, and it sad ' The target voltage for a 24 volt charger for flooded batteries is 2.4 to 2.45 volts per cell, which is 28.8 to 29.4 volts.' To me that seems like a rather large spread to be guessing at.Installation requires no modification to the primary electrical system, resulting in no vehicle warranty issues as you are not connected to the main system. Sterling Power Battery-to-Battery Chargers |